Human Cell-Conditioned Media Produced Under Embryonic-Like Conditions Result in Improved Healing Time After Laser Resurfacing
Springer Science+Business Media, LLC and International Society of Aesthetic Plastic Surgery 2011
Aesth Plast Surg DOI 10.1007/s00266-011-9787-8
M. P. Zimber • J. N. Mansbridge • M. Taylor • T. Stockton •M. Hubka • M. Baumgartner • L. Rheins • K. Hubka •
E. N. Brandt • R. Kellar • G. K. Naughton
Background Laser resurfacing procedures are continuing to grow in popularity as patients select less invasive pro- cedures for rejuvenation of photo-damaged and aging skin. However, although physicians have begun exploring options to aid in postlaser healing, currently available treatments have little clinical evidence to support their use for wounded skin.
Methods When grown under conditions of very low oxygen and suspension, a simulation of the embryonic environment, neonatal cells have been found to produce proteins and growth factors in types and quantities similar to those of fetal cells. The human cell-conditioned media (hCCM) produced by the cells was extracted and formu- lated into a gel to evaluate its efficacy in the healing of postlaser wounds.
Results A split-face clinical evaluation of the material was performed, with 42 subjects undergoing combination ablative and nonablative laser procedures. Three concentrations of the hCCM were tested (9 0.1, 9 1.0, 9 10.0), and a dose–response trend was seen in the blinded physician evaluation, particularly in the assessment of crusting. In addition, transepidermal water loss readings showed a significant difference (p B 0.05), indicating a more rapid return to normal skin barrier function with the active treatment. Histopathologic evaluation of subject biopsies showed reduced inflammation and a more normal epidermal appearance in the active treatment sites.
Conclusions The results of this clinical evaluation sup- port the use of the soluble hCCM produced under embry- onic-like conditions to accelerate wound healing after laser resurfacing procedures. The utility of the 9 10 concen- tration appears to promote more rapid, scarless wound healing after resurfacing procedures and more normal skin recovery.
In the preliminary clinical trial described in this report, 42 healthy volunteers were enrolled in a clinical evaluation to test the efficacy of a formulation containing a human cell conditioned medium in accelerated wound healing after laser resurfacing of the face. The results of this randomized, double-blind, placebo-controlled, split-face study.
support the use of this formulation to accelerate wound healing after facial laser resurfacing. In addition, there was an apparent dose response, with the highest concentration (9 10.0) having the most efficacy.
Use of a controlled-production hCCM that contains products produced under simulated embryonic conditions appears to accelerate wound healing, as seen by the transepidermal water loss data. In addition, the conditioned media appears to promote healing with less inflammation and more normal skin recovery, as noted in the biopsy evaluation. The secreted cytokines, including factors such as KGF and VEGF, aid healing of the skin after the procedure where the outer layer has been compromised by the procedure.